advantages and disadvantages of the serial dilution agar plate technique

The rulings cover 9 square millimeters, and boundary lines of the Neubauer ruling are the center lines of the groups of three.

This Counting Chamber is designed to count bacteria, sperm, and blood platelets.

It contains many aliquots of known volume, during the enumeration process the number of bacterial cells in a single aliquot is counted by using a microscope, and then the total number of cells is determined by mathematically.

For DMC a special slide is used called Petroff-Hausser counting chambers, which consists of a ruled slide and a coverslip.

There are present several steps to accomplish this method, such as direct microscopic counts, use of an electronic cell counter such as the Coulter Counter, chemical methods for estimating cell mass or cellular constituents, turbidimetric measurements for increases in cell mass, and the serial dilution–agar plate method.

This method is also used for the analysis of foods, water, and, in some cases, air for quantitative counting of microorganisms.

Direct Microscopic count (DMC) is a quantitative test and used to enumerate the number of bacterial clumps or somatic cells present in milk.

6 Reference Direct Microscopic Count (DMC)

Advantages and disadvantages of the serial dilution agar plate technique